Coordinated circadian timing through the integration of local inputs in Arabidopsis thaliana

Individual plant cells have a genetic circuit, the circadian clock, that times key processes to the day-night cycle. These clocks are aligned to the day-night cycle by multiple environmental signals that vary across the plant. How does the plant integrate clock rhythms, both within and between organs, to ensure coordinated timing? To address this question, we examined the clock at the sub-tissue level across Arabidopsis thaliana seedlings under multiple environmental conditions and genetic backgrounds. Our results show that the clock runs at different speeds (periods) in each organ, which causes the clock to peak at different times across the plant in both constant environmental conditions and light-dark (LD) cycles. Closer examination reveals that spatial waves of clock gene expression propagate both within and between organs. Using a combination of modeling and experiment, we reveal that these spatial waves are the result of the period differences between organs and local coupling, rather than long-distance signaling. With further experiments we show that the endogenous period differences, and thus the spatial waves, can be generated by the organ specificity of inputs into the clock. We demonstrate this by modulating periods using light and metabolic signals, as well as with genetic perturbations. Our results reveal that plant clocks can be set locally by organ-specific inputs but coordinated globally via spatial waves of clock gene expression.

Data and Resources

Additional Info

Field Value
Author Greenwood, Mark
Last Updated November 20, 2019, 16:52 (UTC)
Created November 4, 2019, 17:56 (UTC)
Article Host Type publisher
Article Is Open Access true
Article License Type cc-by
Article Version Type publishedVersion
Citation Report https://scite.ai/reports/10.1371/journal.pbio.3000407
DOI 10.1371/journal.pbio.3000407
Date Last Updated 2019-10-14T00:30:29.417503
Evidence open (via page says license)
Funder code(s) Gatsby Charitable Foundation (GAT3395/GLC); Biotechnology and Biological Sciences Research Council (BB/K018078/1, BB/K017152/1)
Journal Is Open Access true
Open Access Status gold
PDF URL https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3000407&type=printable
Publisher URL https://doi.org/10.1371/journal.pbio.3000407